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Growth characteristics of cell suspension cultures for secondary metabolite production in Ocimum basilicum L., Ocimum sanctum L and Ocimum gratissimum L

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Author(s): Rebecca Mathew and Deepa Sankar P*

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 10;
Start page: 3323;
Date: 2011;
Original page

Keywords: Cotyledonary leaves | Callus | Cell suspension | Ocimum basilicum L. | Ocimum sanctumL. | Ocimum gratissimum L

ABSTRACT
Cell suspension cultures of Ocimum basilicum L., Ocimum sanctum L. and Ocimum gratissimum L. were established from semi friable calli derived fromcotyledonary leaf explants. Solid and liquid Murashige and Skoog (1962) medium supplemented respectively, with 3% sucrose, 0.5 mg l-1 BA and 1.0 mg l-1 2,4-Dwere used. Cell biomass of suspension cultures was harvested every 48 hours for 8 weeks and growth characteristics were studied in terms of fresh and dry weight.Lag phase was observed till 16th day for the three species. Exponential increase in cell weight was observed from 18th day of culture (log phase) in all three species.Significant biomass accumulation was observed in 14 days for O.basilicum, 32 days for O.sanctum and 28 days for O.gratissimum with the maximum increase beingobserved on 32nd day for O.basilicum, 50th day for O.sanctum and 46th day for O.gratissimum. Cultures entered stationary phase on the 34th day for O.basilicum,52nd day for O.sanctum and 48th day for O.gratissimum.
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