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Growth inhibitory effect of 4-phenyl butyric acid on human gastric cancer cells is associated with cell cycle arrest

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Author(s): Long-Zhu Li | Hong-Xia Deng | Wen-Zhu Lou | Xue-Yan Sun | Meng-Wan Song | Jing Tao | Bing-Xiu Xiao | Jun-Ming Guo

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 18;
Issue: 1;
Start page: 79;
Date: 2012;
Original page

Keywords: Histone deacetylase inhibitor | 4-phenyl butyric acid | Gastric carcinoma | Anticancer effect | Cell cycle | MGC-803 | SGC-7901

ABSTRACT
AIM: To investigate the growth effects of 4-phenyl butyric acid (PBA) on human gastric carcinoma cells and their mechanisms. METHODS: Moderately-differentiated human gastric carcinoma SGC-7901 and lowly-differentiated MGC-803 cells were treated with 5, 10, 20, 40, and 60 ╬╝mol/L PBA for 1-4 d. Cell proliferation was detected using the MTT colorimetric assay. Cell cycle distributions were examined using flow cytometry. RESULTS: The proliferation of gastric carcinoma cells was inhibited by PBA in a dose- and time-dependent fashion. Flow cytometry showed that SGC-7901 cells treated with low concentrations of PBA were arrested at the G0/G1 phase, whereas cells treated with high concentrations of PBA were arrested at the G2/M phase. Although MGC-803 cells treated with low concentrations of PBA were also arrested at the G0/G1 phase, cells treated with high concentrations of PBA were arrested at the S phase. CONCLUSION: The growth inhibitory effect of PBA on gastric cancer cells is associated with alteration of the cell cycle. For moderately-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and G2/M phases. For lowly-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and S phases.
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