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HAIRY ROOT CULTURE OF AN IMPORTANT MEDICINAL PLANT: COLEUS FORSKOHLII

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Author(s): UMA MAHESWARI R, SELVAMURUGAN C, JAYABARATH J, LAKSHMI PRABHA A

Journal: International Journal of Agriculture Sciences
ISSN 0975-3710

Volume: 3;
Issue: 2;
Start page: 82;
Date: 2011;
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Keywords: Agrobacterium rhizogene | Coleus forskohlii | Hairy root | HPLC

ABSTRACT
An experiment was conducted for induction & establishment of Agrobacterium mediated hairy root culture ofColeus forskohlii was developed & co-cultivation on hormone free semisolid MS Medium with B5vitamins.Sterilizationperiod was standardized for the stem & leaf explant using 0.1% bavistin and 0.1% Hgcl2 with varied intervals It resulted inthe emergence of hairy roots from the leaf explant , stem explant after 20th day of infection .The transformation of hairyroots was established and confirmation of forskolin content was done by HPLC. Selection of high forskolin can provide analternative source of large scale production of forskolin.
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