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Has Non-Vascularized Bone Graft Really No Viability? An Experiment Study Of Bone Isograft Using GFP Transgenic Mice

Author(s): Cheng Lin, Yan-pu Liu, De-lin Lei, Xiao-guang Hu, Bao-lin Liu

Journal: Journal of Orthopaedics
ISSN 0972-978X

Volume: 5;
Issue: 3;
Date: 2008;
Original page

Keywords: GFP transgenic mice | isograft | non-vascularized bone graft

Abstract:Purpose: To further understand the viability of the non-vascularized bone graft and the origin of the bone-forming cells, we have developed a novel mouse femur model that permits direct-viewing analysis of bone graft healing.Materials and Methods: Critical size defects were prepared in one side of femur of 15 C57BL/6 mice and repaired by non-vascularized femur with intact periosteum and parts of muscles from isogenous GFP transgenic C57BL/6 mice. Sacrifice was performed 3 days, 1, 2, 3, and 4 weeks after surgery for histological and fluorescence microscopy analysis.Results: Healing process of the transplants is similar to that of bone fracture. The osteoblasts and newly formed trabeculae which were beneath the grafted periosteum showed green fluorescent protein (GFP) expression, which was strongly expressed in the transplanted muscles.Conclusion: These data demonstrate that part of non-vascularized grafted bone can keep viability and has the potential of osteogenesis, indicating an important role for the grafted periosteum and bone in new bone formation
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