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Headspace solid-phase microextraction (HS-SPME) Gas Chromatography Mass Spectrometric (GC-MS) analysis of the volatile aroma components of Gentiana kurroo Royle.

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Author(s): Bilal A. Wani1,D. Ramamoorthy1, Manzoor A. Rather2,*Bashir A. Ganai3, Akbar Masood3, M. A. Zargar3 and IshfakH. Wani3

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 9;
Start page: 2943;
Date: 2011;
Original page

Keywords: Gentiana kurroo Royle | HS-SPME | GC-MS | Kashmir Himalaya | Oxygenated monoterpenes | a-terpinyl acetate.

ABSTRACT
Headspace solid-phase micro extraction (HS-SPME) coupled with Gas Chromatography Mass Spectrometry (GC-MS) has been used in the present study to isolateand identify volatile aroma components from the aerial parts of Gentiana kurroo Royle, a critically endangered and endemic medicinal plant growing wild inKashmir Himalaya (India). The present study is the first report on the composition of volatile aroma of this plant species. The analysis has led to the identificationof 16 volatile components constituting 99.3% of the total HS-SPME extract. The chemical composition of the HS-SPME extract of G. kurroo comprises mainlyof sulphur containing compounds constituting 36.1% of the total identified components. Oxygenated monoterpenes constitute 31.3%, oxygenated diterpenes12.6%, aldehydes 11.5% and alcohols 3.9%. The major identified components were a-terpinyl acetate (23.5%), 2-ethyl furan (17.5%), dimethyl sulphide (14.7%),methandriol (12.6%), 1, 8-cineole (7.8%), 3-methyl butanal (4.4%) and pentanal (3.2%). The characteristic aroma of the dried leaves of G. kurroo might bebecause of high content of sulphur compounds.
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