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Headspace Solid Phase Microextraction (HS SPME) Gas Chromatography Mass Spectrometric Analysis of the Volatile Constituents of Cannabis sativa L. From Kashmir.

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Author(s): Manzoor A. Rather a *, Bilal A. Dar a, Shahnawaz N. Sofi a, Tauheeda Hassan b, Nasir Ali a, Ashiq H. Lone a, Abdul S. Shawl a, Wajahat A. Shah c, M. A. Qurishi c and Poonam Prakash d.

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 8;
Start page: 2651;
Date: 2011;
Original page

Keywords: Cannabis sativa | Cannabiaceae | HS-SPME | GC-MS | trans-caryophyllene | a- humulene.

ABSTRACT
Headspace Solid-phase micro extraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS) has been used to isolate and identify the volatile compounds from the leaves of Cannabis sativa growing in Kashmir. The analysis led to the identification of 17 volatile components constituting 94.8 % of the total identified components. The chemical composition of the SPME extract from the leaves of C. sativa comprised mainly of sesquiterpenehydrocarbons (64.3%), monoterpene hydrocarbons (18.4%) and alcohols (10.3%). The major components identified in the HS-SPME extract were transcaryophyllene (36.9%), a-humulene (16.2%), a-pinene (10.7%), 3-hexen-1-ol-acetate (6.2%) and ß-pinene (4.2%). The current study is the first report involving rapid analysis of volatile components of C. sativa by HS-SPME.
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