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Hepatic non-parenchymal cells and extracellular matrix participate in oval cell-mediated liver regeneration

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Author(s): Wei Zhang, Xiao-Ping Chen, Wan-Guang Zhang, Feng Zhang, Shuai Xiang, Han-Hua Dong, Lei Zhang

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 15;
Issue: 5;
Start page: 552;
Date: 2009;
Original page

Keywords: Oval cells | Liver regeneration | Extracellular matrix | Hepatic stellate cells | Kupffer cells

ABSTRACT
AIM: To elucidate the interaction between non-parenchymal cells, extracellular matrix and oval cells during the restituting process of liver injury induced by partial hepatectomy (PH).METHODS: We examined the localization of oval cells, non-parenchymal cells, and the extracellular matrix components using immunohistochemical and double immunofluorescent analysis during the proliferation and differentiation of oval cells in N-2-acetylaminofluorene (2-AAF)/PH rat model.RESULTS: By day 2 after PH, small oval cells began to proliferate around the portal area. Most of stellate cells and laminin were present along the hepatic sinusoids in the periportal area. Kupffer cells and fibronectin markedly increased in the whole hepatic lobule. From day 4 to 9, oval cells spread further into hepatic parenchyma, closely associated with stellate cells, fibronectin and laminin. Kupffer cells admixed with oval cells by day 6 and then decreased in the periportal zone. From day 12 to 15, most of hepatic stellate cells (HSCs), laminin and fibronectin located around the small hepatocyte nodus, and minority of them appeared in the nodus. Kupffer cells were mainly limited in the pericentral sinusoids. After day 18, the normal liver lobule structures began to recover.CONCLUSION: Local hepatic microenvironment may participate in the oval cell-mediated liver regeneration through the cell-cell and cell-matrix interactions.
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