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Heterogenous stem cells from nonsorted versus sorted mononuclear cell from bone marrow: FACS based approach

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Author(s): S.Indumathi

Journal: Biology and Medicine
ISSN 0974-8369

Volume: 3;
Issue: 3;
Start page: 13;
Date: 2011;
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Keywords: Bone marrow | flow cytometry | sorting | mononuclear cell | HSC | MSC

ABSTRACT
The widely accepted source of stem cell for clinical transplant is the infusion of mononuclear cell (MNC) from bonemarrow. The disadvantages of MNC infusion pose a threat to the world of regenerative medicine because of its vastheterogeneity, granulocyte interface and age. Thus, finding an alternative on improvisation of MNC therapy is ofutmost importance. Thereby, we hypothesized an alternative approach by sorting of lymphocytes and monocytesusing an FACS based purification method. To confirm our speculation, we enumerated various stem cell populationsin non-sorted as well as sorted MNC from bone marrow. We found that sorted lymphocytes and monocytes possesshigh percentage of stem cells when compared to non-sorted MNC. Thus, it is concluded that sorted lymphocytes andmonocytes cocktail from bone marrow might be an efficient alternative approach in cell based therapies. However,further investigations on these sorted cells will bring this work closer to clinical applications.
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