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A High-Performance Liquid Chromatographic Assay for the Determination of Losartan in Plasma

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Author(s): Hassan Jalalizadeh | Effat Souri | Hassan Farsam | Mehdi Ansari

Journal: Iranian Journal of Pharmacology and Therapeutics
ISSN 1735-2657

Volume: 2;
Issue: 1;
Start page: 18;
Date: 2003;
Original page

Keywords: Losartan | Plasma | Extraction | HPLC analysis

ABSTRACT
A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium diclofenac was selected as internal standard. Excellent linearity between the peak area ratios and losartan concentrations over the range of 2-200 ng/mL of plasma was observed. The limit of determination with UV detection at 225 nm, with a CV < 5% was 2 ng/mL in 500 L of plasma sample. The assay was rapid, safe and reliable for use in pharmacokinetic studies of losartan in human being.
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