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High-Performance Liquid Chromatography: An Overview

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Author(s): Tandia N | Singh SK | Kumar N | Singh S

Journal: International Journal For Pharmaceutical Research Scholars
ISSN 2277-7873

Volume: 2;
Issue: 4;
Start page: 1;
Date: 2013;
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Keywords: HPLC | Sample | Chromatography | Instrumentation technique

ABSTRACT
Chromatography is considered extremely powerful separation technique for variety of samples. Thecommon feature of all these techniques is that the different components in a sample mixture aredistributed between two phases, one of which remains stationary while the other the mobile phase, runsthrough the interstices or over the surface of the fixed phase. The movement of the mobile phase resultsin differential migration of the sample components. High performance thin layer chromatography is aninvaluable quality assessment tool for the evaluation of botanical materials and the equally suitable forqualitative and quantitative analysis of biological samples which are of particular interest to biologists,biochemists, hematologists, immunologists, medical diagnosticians, and molecular biologists. It allowsfor the analysis of a broad number of compounds efficiently and cost effectively. Over three decades ofdevelopment in HPLC have given us a well understood separation tool capable of very reliable results.Refinements in HPLC column technology have lead to high resolution separations, fast analysis, andhigh sensitivity. However, to obtain all the benefits that HPLC can offer, careful attention must be paidto that part of the liquid flow system that carries the sample.
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