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High abundances of neurotrophin 3 in atopic dermatitis mast cell

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Author(s): Quarcoo David | Fischer Tanja | Peckenschneider Nora | Groneberg David | Welker Pia

Journal: Journal of Occupational Medicine and Toxicology
ISSN 1745-6673

Volume: 4;
Issue: 1;
Start page: 8;
Date: 2009;
Original page

ABSTRACT
Abstract Background Neurotrophin 3 (NT-3) is a member of the neurotrophin family, a group of related proteins that are known to regulate neuro-immune interactions in allergic diseases. Their cellular sources and role in the recruitment of mast cell precursors in atopic dermatitis have not been characterized in detail so far. Objective Characterize NT-3 on a transcriptional and translational level in individuals with atopic dermatitis with special focus on mast cells. Methods To meet this objective NT-3 levels in the serum of AD patients were measured, the effect of NT-3 on keratinocytes was evaluated and the gene expression and regulation assessed using ELISA, immunohistochemistry and RNA quantification. Results Systemic levels of NT-3 were found to be higher in individuals with AD as compared to healthy controls. A distinct genetic expression was found in the various cells of the skin. In lesional mast cells of individuals with atopic dermatitis an increased amount of NT-3 was apparent. Functional in vitro experiments demonstrated that NT-3 stimulation led to a suppression of IL-8 secretion by HaCat cells. Conclusion These findings could imply a role for NT-3 in the pathogenesis of allergic skin diseases.

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Tangokurs Rapperswil-Jona

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