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High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells

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Author(s): Xiao-Nan Cui, Jian-Wu Tang, Bo Song, Bo Wang, Shan-Yan Chen, Li Hou

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 15;
Issue: 48;
Start page: 6117;
Date: 2009;
Original page

Keywords: Osteoglycin | Transfection | Hepatocellular carcinoma | Neoplasm metastasis | Genes | Gelatinases

ABSTRACT
AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells.METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis.RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo.CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.
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