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HPLC Method Development for Glucosamine Sulphate and Diacerein Formulation

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Author(s): Useni Reddy Mallu1 K. Hussain Reddy1, Varaprasad Bobbarala2* and Somasekhar Penumajji3

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 3;
Issue: 2;
Start page: 361;
Date: 2010;
Original page

Keywords: High performance liquid chromatography (HPLC) | Glucosamine | Diacerein.

ABSTRACT
A simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Glucosamine sulphate and Diacerein for assay was developed. Glucosamine and Diacerein were baseline separated and quantitated on C8 reversed phase column (4.6×250mm, 5.0µm), using a mobile phase composed of a phosphate buffer-acetonitrile (55:45v/v, pH 3.0) delivered at a flow rate of 0.6mL/min, and with UV detection ( l excitation = 195nm). The method was proven to be linear over a Glucosamine concentration range of 84 to 504µg/mL with a mean correlation coefficient of 0.9999 and a Diacerein concentration range of 5.6 to 33.6µg/mL with a mean correlation coefficient of 0.9998.
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