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HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration

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Author(s): Kadumi Inoue | Kei-ichi Kitahara | Yoshihiro Aikawa | Sadao Arai | Takako Masuda-Hanada

Journal: Molecules
ISSN 1420-3049

Volume: 16;
Issue: 7;
Start page: 5905;
Date: 2011;
Original page

Keywords: carbohydrate | aldose | aldopentose | aldohexose | high-performance anion-exchange chromatography

ABSTRACT
To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D6 stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N’,N’-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pKa values, but also the chemical structures of the cyclic aldoses.
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Tango Jona
Tangokurs Rapperswil-Jona