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Identification and Characterization of gtf, norB, and trx Genes in Flavobacterium Columnare

Author(s): Yinfeng Zhang | Covadonga R. Arias

Journal: Journal of Microbial & Biochemical Technology
ISSN 1948-5948

Volume: 01;
Issue: 01;
Start page: 064;
Date: 2009;
Original page

Keywords: Flavobacterium columnare | gtf | norB | trx gene expression | Iron-limited | Catfish skin explants

Three genes from a shotgun genomic library of Flavobacteriumcolumnare virulent strain ALG-00-530 were identified,characterized, and selected for differential expressionanalysis based on sequence similarity to putative virulencegenes from related species. These genes were:glycosyltransferase (gtf), nitric oxide reductase (norB) andthioredoxin (trx). A collection of 30 F. columnare strains,including strains from genomovars I and II, were testedfor the presence of these genes. Distribution patterns ofgtf, norB, and trx across the species were not uniform.Nucleotide sequence variation was observed betweengenomovars for each gene; however, strains within thesame genomovar shared identical gene sequences. Ninestrains of F. columnare, from both genomovars, were chosenfor gene expression analysis. The expression profileof these genes varied when selected strains were grown invitro under identical conditions. ALG-00-530, a high virulentstrain, was chosen for gene expression comparisonunder standard growing conditions, iron-limited conditionsand in the presence of skin explants from channel catfish.NorB, and trx gene expression levels varied when ALG-00-530 was incubated under different conditions.
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