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Identification of Insect Cell Lines from 8 Lepidopteran species by DNA Amplification Fingerprinting

Author(s): Sadeq Khalid Nessr Alhag | Yao Han Chao | Peng Jian Xin

Journal: Journal of Applied Sciences
ISSN 1812-5654

Volume: 7;
Issue: 24;
Start page: 4040;
Date: 2007;
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Keywords: fingerprint | | DNA | Amplification | insect cell lines | PCR

DNA Amplification Fingerprinting (DAF) with arbitrarily selected primers was used to obtain DNA fingerprint profiles to distinguish among 8 lepidopteran insect cell lines. The fingerprinting pattern is a stable characteristic of the cell line because high and low passages generated the same profile. The DNA from each cell line was amplified and PCR products were analyzed by agarose gel electrophoresis. All cell lines could be distinguished from each other with following exception: Bombyx mori (Bm-e-HNU5) produced the same profile as Laphygma exigua (Le-H-HNU7) also Spodoptera exigua (UCR-SE-1C) produced identical patterns to Spodoptera litura (SL-ZSU-1). DAF will serve as an additional, valuable and reliable technique for the identification of insect cell lines.
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