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IDENTIFICATION OF OILSEED RAPE CULTIVARS USING AFLP MARKERS

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Author(s): V ČURN | J OVESNÁ | L SÁKOVÁ | R SOBOTKA

Journal: Journal of Central European Agriculture
ISSN 1332-9049

Volume: 3;
Issue: 4;
Start page: 285;
Date: 2003;
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Keywords: aflp | oilseed rape | brassica napus

ABSTRACT
A new type of molecular marker, fluorescence-based AFLP, was evaluated for its ability to identify oilseed rape cultivars. Each of the six tested AFLP combinations detected polymorphisms, the best combination (MCAA/ E-ACT) had 26% of polymorphic peaks from a total of 90 peaks and could distinguish analysed cultivars, and 4 out of 5 DH lines. The results presented here show that florescence-based AFLP was, for the purposes of oil seed rape cultivar fingerprinting, a very suitable approach.
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