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Immobilization of the Protease of Carica papaya on DEAE-cellulose

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Author(s): H. Bokhari | M. R. Khan

Journal: Pakistan Journal of Chemistry
ISSN 2220-2625

Volume: 2;
Issue: 4;
Start page: 1;
Date: 2012;
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Keywords: DEAE-cellulose | Immobilized protease | Carica papaya | Proteolysis

ABSTRACT
Proteolytic enzymes can immobilize on different materials to constitute systems for continuous hydrolysis of protein substrates.They are mostly immobilized by binding some matrices filled in chromatographic columns and continuous proteolysis isaccomplished by subsequent passing of the buffered substrates through the column bed from top to bottom and collecting ofhydrolyzed products eluting out of the column from the bottom. Immobilization of the protease of Carica Papaya was carried outon DEAE-cellulose. The percentage of immobilization was found to be 67%.The life span of the enzyme immobilized on DEAE-cellulose was 30 days.
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