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Immune Responses to Antigens of in vitro Reared Echinococcus granulosus Adult Worms in Balb/c Mice

Author(s): Hamid Reza Rahimi | Bahador Sarkari | Tahereh Mohammadzadeh | Seyed Mahmoud Sadjjadi

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 4;
Start page: 236;
Date: 2011;
Original page

Keywords: Antigen | Echinococcus granulosus | Excretory | Mice

Background: Cystic echinococcosis (CE), also known as echinococcosis/hydatidosis, isone of the most important parasitic diseases in the world. It enhances both humoral andcellular (Th1 and Th2) responses in infected host. Different antigens of the worm mayfavor the Th1 or Th2 immune responses in CE patients. Objective: To evaluate the humoraland cellular immune responses of Balb/c mice against the crude and excretory/secretory (E/S) antigens of in vitro reared Echinococcus granulosus adult worms.Methods: A total of 20 Balb/c mice divided into 5 groups of 4 mice each. Three groupsof mice (n=4) were immunized with crude, E/S and an immunodominant antigen of invitro reared Echinococcus granulosus adult worms on day 1 and 28. The fourth and thefifth groups were negative control groups and received PBS plus adjuvant, or nothing,respectively. Two weeks after the second injection, the mice were killed and their bloodwas collected for determining antibody responses, and their spleens were employed forproliferation assay. Total IgG were measured by indirect ELISA. Spleen cells of immunizedmice were cultivated and exposed to different antigens of adult worms includingE/S and crude antigens. Level of IFN-γ, IL-12, IL-4 and IL-10 were measured in therecovered cell culture supernatants by capture ELISA. Results: Total IgG assay showedthe highest level of antibody produced in mice immunized with crude antigens. Proliferationassay showed a statistically significant production of cytokines in the mice immunizedwith crude antigens (p

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