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Immunohistochemical and ultrastructural characteristics of results of surgical treatment of patients with ІІІ degree chronic ischemia of lower limb

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Author(s): Shkuropat V.M. | Tverdokhleb I.V. | Drjuk M.F.

Journal: Морфологія
ISSN 1997-9665

Volume: 3;
Issue: 3;
Start page: 142;
Date: 2009;
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Keywords: ischemia of the lower limb | revascularization | postoperative period | immunohistochemistry | VEGF | CD34 | electron microscopy

ABSTRACT
The purpose of research was the immunohistochemical and ultrastructural analysis of anterior tibial muscle in patients with ІІІ degree chronic ischemia of lower limb after direct, indirect and composite revascularization in near-term and long-term postoperative periods. Patients have been divided into three groups: 1) 37 patients after femoral-tibial reconstruction; 2) 57 patients after indirect revascularization with autotransplantation of bone marrow; 3) 50 patients after composite revascularization of distal part of lower limb. The observation was carried out in the near-term postoperative period and in 2 years. Immunohistochemical investigation of markers VEGF and CD34 expression and ultrastructural study of tissue sam-plings of anterior tibial muscle taken between superior and middle one thirds was carried out. It have been determined that direct revascularization causes the significant improvement in immunohistochemical characteristics of anterior tibial muscle on VEGF and CD34 expression both in ultrastructure of muscle fibers in all age groups during 6 months after operation, however does not provide the stabilization of positive changes in long-term postoperative period. Indirect revascularization does not change significally structurally-functional condition of components of muscle in near-term postoperative period, however causes the stable normalization of parameters of tissue components due to initiation of neovasculogenesis at patients till 75 years. Composite revascularization allows to receive near-term normalizing effect concerning the studied immunohis-tochemical and ultrastructural criteria and to provide its stabilization in the long-term postoperative period.
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