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Immunoinhibitory Effect of Teuclatriol a Guaiane Sesquiterpene from Salvia mirzayanii

Author(s): Akram Ziaei | Zahra Amirghofran | Josef Zapp | Mohammad Ramezani

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 4;
Start page: 226;
Date: 2011;
Original page

Keywords: Immunomodulatory | Salvia mirzayanii | Sesquiterpenes

Background: Salvia mirzayanii, a native plant to Iran, is shown to have immunomodulatoryeffects on lymphocyte proliferation. Objective: To identify the bioactive immunomodulatorycompound(s) present in S. mirzayanii. Methods: The crude extract wasfractionated to five fractions in two steps using different solvents. The fractions weresubjected to bioassay-guided fractionation. All the fractions were tested for bioactivityon human activated-peripheral blood lymphocytes (PBLs) using cell proliferation assay.Results: The methanol fraction (Fr. M) showed the highest inhibitory effect on PBLscompared to other fractions. Fr. M was applied on a gravity column chromatography forfurther fractionation. Resultant fractions, demonstrated inhibitory effects at higher concentrations.Fr. 4 with an 18.9 ± 0.2% inhibitory activity at 200 μg/ml and with thehighest quantity was applied on preparative TLC plates for further purification. The finalpurified compound was identified as teuclatriol, a guaiane sesquiterpene, by NMRanalysis. This compound showed a significant anti-proliferative effect on human activated-peripheral blood lymphocytes (IC50, 72.8 ± 5.4 μg/ml). Conclusion: Teuclatriolwas found to be one of the compounds responsible for the immunoinhibitory effect ofSalvia mirzayanii. We suggest further studies on teuclatriol, exploring its mechanism ofaction as an immunomodulatory compound.

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