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Immunomodulatory activity of a Chinese herbal drug Yi Shen Juan Bi in adjuvant arthritis

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Author(s): Perera Pathirage | Li Yunman | Peng Cheng | Fang Weirong | Han Caifeng

Journal: Indian Journal of Pharmacology
ISSN 0253-7613

Volume: 42;
Issue: 2;
Start page: 65;
Date: 2010;
Original page

Keywords: Synoviocytes | TNF-α | IL-1β | caspase-3

ABSTRACT
Objective: To investigate the immunomodulating mechanisms of a Chinese herbal medicine Yi Shen Juan Bi (YJB) in treatment of adjuvant arthritis (AA) in rats. Materials and Methods: Levels of serum tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β) were measured by the Enzyme-Linked Immunosorbent Assay (ELISA). Expression of TNF-α mRNA and IL-1β mRNA in synovial cells was measured with the semi-quantitative technique of reverse transcription-polymerase chain reaction (RT-PCR), while caspase-3 was examined by western blot analysis. Results: The administration of YJB significantly decreased the production of serum TNF-α and IL-1β. It also decreased significantly the TNF-α mRNA, IL-1β mRNA, and caspase-3 expression in synoviocytes. Conclusions: YJB produces the immunomodulatory effects by downregulating the over-activated cytokines, while it activates caspase-3, which is the key executioner of apoptosis in the immune system. This may be the one of the underlying mechanisms that explains how YJB treats the rheumatoid arthritis.
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