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"Immunoperoxidase" an essential modality of immunohistochemistry

Author(s): Gupta P | Rapose Alwyn

Journal: Indian Journal of Dermatology, Venereology and Leprology
ISSN 0378-6323

Volume: 63;
Issue: 6;
Start page: 361;
Date: 1997;
Original page

Keywords: Immunoperoxidase (IP) | Immune deposits | Standardization | Optimum dilution

The immunoperoxide (IP) technique can be used for the detection of immune deposits in various tissue pathologies. This article focusses on the standardization of the reagents anti IgG, anti IgA and anti C3C which are used to target these immune deposits. Lymph node tissue was used as substrate for standardization of anti IgG and anti IgM. For anti IgA, intestine was used while for anti C3C kidney tissue rapidly proliferating glomerulo nephritis - RPGN was utilized. Sensitivity of the technique was found to be high. Also the results on paraffin section were excellent and could be interpreted using simple light microscope. It is concluded that once standardization is achieved, IP can be used as an essential modality in the detection of immune deposits in a wide variety of immunologically mediated dermatologic disorders.
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