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Impairment of inhibition of HSP70 by Helicobacter pylori in human epithelial MKN7 cell line

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Author(s): Aneta Targosz | Agata Ptak-Belowska | Urszula Szczyrk | Gracjana Krzysiek-Mączka | Wiesław W. Pawlik | Tomasz Brzozowski

Journal: Polish Gastroenterology
ISSN 1232-9886

Volume: 17;
Issue: 3;
Start page: 181;
Date: 2010;
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Keywords: Helicobacter pylori | HSP70

ABSTRACT
Introduction: Heat shock proteins (HSPs) are considered as a key component of signaling pathways involved in gastric mucosal defense but their interaction with Helicobacter pylori (Hp) remains unknown. Aim of the study: To compare the effect of cell incubation with bacteria applied alone or in combination with exogenous CagA protein on the expression mRNA of HSP70 in MKN7 cells exposed to the Hp strain expressing cagA and vacA. Material and methods: The MKN7 cells were incubated in standard medium RPMI 1640 in the presence of Hp cagA vacA positive (1×109 CFU per dish) without or with the recombinant protein CagA. After incubation the cells were harvested and total cellular RNA was isolated. The expression of mRNA was determined by RT-PCR. Results: Hp inhibits expression of HSP70 in MKN7 cells and the addition of exogenous CagA causes significant inhibition of HSP70 as compared to that without CagA. Conclusion: Our finding that Hp cagA- and vacA-positive inhibits expression of HSP70 suggests that downregulation of HSP70 might be a primary cause of Hp -associated mucosal damage and apoptosis, thus reinforcing the notion that the induction of HSP family proteins may constitute a novel therapeutic approach for prevention and treatment of this condition.
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