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Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1

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Author(s): Milić Nenad | Nišavić Jakov | Ašanin Ružica | Knežević Aleksandra | Ašanin Jelena | Vidanović D. | Šekler Milanko

Journal: Veterinarski Glasnik
ISSN 0350-2457

Volume: 64;
Issue: 3-4;
Start page: 159;
Date: 2010;
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Keywords: bovine herpesvirus 1 | PCR | Real-Time PCR

ABSTRACT
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
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