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Improvement of Carboxymethyl Cellulase and Xylanase Production by Alginate Immobilized Trichoderma harzianum

Author(s): Idress H. Attitalla | Baharuddin Salleh

Journal: Biotechnology
ISSN 1682-296X

Volume: 9;
Issue: 4;
Start page: 529;
Date: 2010;
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Keywords: carboxymethyl cellulase and xylanase production | Ca-alginate | immobilization | biocontrol | Trichoderma harzianum

Conidia of two isolates of Trichoderma harzianum (T7 and T8) (Rifai) were formulated to make alginate pellets with or without xylan or CMC as a food-base material. The formulations were compared for their ability of in vitro carboxymethycellulase and xylanase production with free fungal spore suspensions. Conidia entrapped in alginate with or without adjuvant showed high production of enzymes even when repeated 4 times. The addition of adjuvant significantly enhanced the enzyme production. Alginate concentration and surface area of the beads affected the enzyme production. The optimum initial pH and incubation temperature were pH = 5-7 and 35°C for CMC-ase and pH = 5 and 30°C for xylanase. Alginate encapsulated Trichoderma not only prolonged the metabolic activity of the entrapped organism, but also it promotes slow release of microbial spores into the medium for successful enzyme production.
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