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INDUCTION OF CALLUS FORMATION FROM DIFFERENT PARTS OF Citrus grandis (OSBECK) FLOWERS

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Author(s): Zarina Binti Zakaria | Suhana Binti Zakaria | Abd Hamid Bin Khalid | Mohd Azlan Bin Mohd Ishak

Journal: BIOTROPIA : the Southeast Asian Journal of Tropical Biology
ISSN 0215-6334

Volume: 17;
Issue: 1;
Start page: 1;
Date: 2010;
Original page

Keywords: Citrus grandis | callus induction | flowers | auxin | cytokinin

ABSTRACT
The objectives of the present work were to study the capability of pomelo's floral tissues to produce callus and to investigate the influence of plant growth regulators on callus induction and development. Various parts of flower namely petal, sepal, style, ovary, pistil and cup base were cultured onto Murashige and Skoog (MS) basal medium supplemented with different levels of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and 6-Furfurylaminopurine (kinetin). It was found that different parts of flowers favored different levels of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. A 50 ± 2.3%explants from style and pistil had initiated callus when cultured on MS media supplemented with 0.05 mg/l and 0.10 mg/l BAP, respectively. On the other hand, sucrose agar (SA) media alone had managed to induce callus formation from almost every part of the flowers with a success rate between 7.69 ± 0.32 to 50 ± 3.18%. The uncut part of flowers initiated high percentage of callus (14.6 ± 0.35%) as shown in the study on petal.
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