Academic Journals Database
Disseminating quality controlled scientific knowledge

Induction of microRNA-214-5p in human and rodent liver fibrosis

Author(s): Iizuka Masashi | Ogawa Tomohiro | Enomoto Masaru | Motoyama Hiroyuki | Yoshizato Katsutoshi | Ikeda Kazuo | Kawada Norifumi

Journal: Fibrogenesis & Tissue Repair
ISSN 1755-1536

Volume: 5;
Issue: 1;
Start page: 12;
Date: 2012;
Original page

Keywords: Collagen | Hepatocyte | Non-coding RNA | Stellate cell | Transforming growth factor-β

Abstract Background miRNAs are non-coding RNAs that regulate gene expression in a wide range of biological contexts, including a variety of diseases. The present study clarified the role of miR-214-5p in hepatic fibrogenesis using human clinical tissue samples, livers from rodent models, and cultured hepatic stellate cells. Methods The expression of miR-214-5p and genes that are involved in liver fibrosis were analyzed in hepatitis C virus-infected human livers, rodent fibrotic livers, a human stellate cell line (LX-2), and the cells from intact mouse livers using real-time PCR. The effect of miR-214-5p overexpression in LX-2 cells on cell function was investigated. Twist-1 expression in the liver tissues of mouse models and primary-cultured stellate cells was also analyzed. Results miR-214-5p was upregulated in human and mouse livers in a fibrosis progression–dependent manner. miR-214-5p expression increased during the culture-dependent activation of mouse primary stellate cells and was significantly higher in stellate cells than in hepatocytes. The overexpression of miR-214-5p in LX-2 cells increased the expression of fibrosis-related genes, such as matrix metalloproteinase (MMP)-2, MMP-9, α-smooth muscle actin, and transforming growth factor (TGF)-β1. TGF-β stimulation induced miR-214-5p in LX-2 cells. Twist-1 was increased in fibrotic mouse livers and induced during mouse stellate cell activation. Conclusion miR-214-5p may play crucial roles in the activation of stellate cells and the progression of liver fibrosis. Twist-1 may regulate miR-214-5p expression in the liver, particularly in stellate cells.
Affiliate Program      Why do you need a reservation system?