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The influence of anthracosis and p16ink4a gene abberant methylation to the oncogenesis and progression of small pulmonary adenocarcinoma

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Author(s): Daye WANG | Jue WANG | Yong LI

Journal: Chinese Journal of Lung Cancer
ISSN 1009-3419

Volume: 11;
Issue: 4;
Start page: 542;
Date: 2008;
Original page

Keywords: Pneumoconiosis | p16 genes | Methylation | Lung neoplasms

ABSTRACT
Background and objective Anthracosis is black dust matter deposition in the pulmonary parenchyma, which can cause bronchial deformity and destruction. Previously reported, anthracosis is closely correlated to the oncogenesis and progression of small pulmonary adenocarcinoma and p16ink4a gene aberrant methylation was closely associated with lung carcinogenesis. In this study, we want to characterize the influence of anthracosis and p16ink4a gene aberrant methylation on small adenocarcinoma. Methods DNA was bisulfite modified and then Methylation Specific PCR was used to detect p16ink4a gene aberrant methylation, and black dust matter was extracted from lung tissues, the absolute absorbance (A) detected by densitometry was defined as anthracotic index (AI). The histopathologic diagnosis was according to Noguchi's classification for small pulmonary adenocarcinoma. Results For heavy smokers, the mean AI was significantly higher than that of nonsmokers (P=0.005) and the frequency of p16ink4a gene aberrant methylation was also significantly higher than that of nonsmokers (P=0.023). The frequency of p16ink4a gene aberrant methylation of early stage small adenocarcinoma was lower than that of advanced and poor differentiated small adenocarcinoma, otherwise p16ink4a protein expression of early stage small adenocarcinoma was significantly higher than that of poor differentiated small adenocarcinoma (P=0.032). Conclusion AI and p16ink4a gene aberrant methylation detection could be used as a combined potential biomarker of small adenocarcinoma.  
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