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The Influence of Inhibiting Angiopoietin-2 on the Biological Characteristics of 
Bronchogenic Adenocarcinoma

Author(s): Bailing LI | Guanxin ZHANG | Xiaolei HOU | Yang YUAN | Xiaohong LIU | Dejun GONG | Shengdong HUANG

Journal: Chinese Journal of Lung Cancer
ISSN 1009-3419

Volume: 14;
Issue: 7;
Start page: 561;
Date: 2011;
Original page

Keywords: Angiopoietin-2 | A549 cell strain | Adeno-associated virus | RNA interference

Background and objective It is well-known that angiopoietin-2 (Ang-2) plays an important role in the formation of the blood vascular system. Angiopoietin is involved in many diseases about angiogenesis such as tumor, so may have great prospects for the treatment of these diseases. The aim of this study is to evaluate the influence of inhibiting Ang-2 via adeno-associated virus induced RNA interference (RNAi) on the biological characteristics of bronchogenic adenocarcinoma. Methods AAV-Ang-2shRNA driven by H1 promoter was constructed to transfect A549 cell line. Normal and AAV-Null cell line were utilized in the control groups. The influence of RNAi on Ang-2 expression as well as the growth rate, tumorigenic efficiency, proliferation rate, apoptosis, and microvessel density of A549 cell line were analyzed. Results In vitro experiment indicated that the Ang-2 expression level (P
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