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The influence of molecular weight of polyethylene glycol on separation and purification of pectinases from Penicillium cyclopium in aqueous two-phase system

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Author(s): Prodanović Jelena M. | Antov Mirjana G.

Journal: Acta Periodica Technologica
ISSN 1450-7188

Volume: 2008;
Issue: 39;
Start page: 193;
Date: 2008;
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Keywords: aqueous two-phase system | bioseparation | pectinases

ABSTRACT
In this study the possibility of the partitioning and purification of pectinases from Penicillium cyclopium by their partitioning in polymer/polymer and polymer/salt aqueous two-phase systems was investigated. In the system with 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500 000/85% (w/w) crude enzyme, the highest values for partitioning parameters were achieved - the partition coefficient was 2.11, followed by the top phase yield of 85.68% and purification factor 1.28 for the endo-pectinase activity. The partition coefficient, yield in the top phase and purification factor for the exo-pectinase activity in the same system were 1.89, 84.28% and 3.82, respectively. In the system with 10% (w/w) polyethylene glycol 6000/15% (w/w) (NH4)2SO4/75% (w/w) crude enzyme purification factor 37.85 for exo-pectinase, and 19.52 for endo-pectinase in the bottom phase were obtained.
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Tango Jona
Tangokurs Rapperswil-Jona