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The influence of the season and culture medium on micropropagation of two intergeneric Fragaria x Potentilla varieties

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Author(s): Anca Nicoleta ŞUŢAN | Aurel POPESCU | Valentina ISAC

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVII;
Issue: 1;
Start page: 190;
Date: 2010;
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Keywords: in vitro culture | season | culture media | intergeneric hybrids | Fragaria x Potentilla

ABSTRACT
In order to develop a protocol for high efficiency in vitro propagation of two intergeneric Fragaria x Potentilla varieties, ‘Serenata’ and ‘Pink Panda’ respectively, the influence of season on the rate of multiplication was investigated in shoot cultures on Murashige and Skoog (MS) and Lee and Fossard (LF) media, supplemented with different combinations of growth regulators. In vitro performance of explants indicated a positive correlation between shoot proliferation and season in both genotypes of ornamental strawberry. The mean number of shoots formed per explant was higher when ‘Serenata’ and ‘Pink Panda’ varieties were subcultured on MS or LF media, in the active growing season, irrespective of the culture medium composition. In both ornamental strawberry varieties, the mean number of shoots formed per explant was slightly higher when subcultured on MS medium, in the spring and summer season, as compared to LF medium, which was proven to be the most effective in the cold season.
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