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Influence of short- and long-term exposition to Roundup on the spontaneous and hormonally stimulated LH secretion and gills condition in Prussian carp (Carassius gibelio)

Author(s): Grzegorz Gosiewski | Magdalena Socha | Elżbieta Nowak | Jacek Kuchinka | Mirosława Sokołowska-Mikołajczyk

Journal: Advances in Environmental Sciences
ISSN 2066-7620

Volume: 4;
Issue: 3;
Start page: 146;
Date: 2012;
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Keywords: gills | LH | Prussian carp | Roundup

Roundup is the most extensively used herbicide in world agriculture. In the present study weexamined the influence of Roundup at the concentration of 15 mg L-1on luteinizing hormone (LH)secretion and reproduction of Prussian carp females. Experiment was conducted at the University ofAgriculture, Department of Ichthyobiology and Fisheries in Kraków, Poland from May to October 2010.Control fish were kept in Roundup–free water. Short-term exposition lasted 10 days, and long-termexposition 6 months. At the end of both experiments samples of blood were collected and all fish wereinjected with salmon GnRH analogue (D-Arg6 sGnRH-A, 10 μg kg-1of b.w.) and pimozide (dopamineantagonist, 5 mg kg-1of b.w). After injection blood samples were collected at 6, 12 and 24 hours. LHlevels were measured by the ELISA. For histopathological analyses gill samples were stained with H&Emethod. Analysis of LH concentrations did not show any significant differences between LH levels neitherin experimental nor control group at both times of exposition. However the pathological changes in gillsin fish from experimental groups in short and long-term exposition were found.
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