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Insight into trichomonas vaginalis genome evolution through metabolic pathways comparison

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Author(s): Satendra Singh | Gurmit Singh | Nitin Sagar | Pramod Kumar Yadav | Prashant A Jain | Budhayash Gautam | Gulshan Wadhwa

Journal: Bioinformation
ISSN 0973-2063

Volume: 8;
Issue: 4;
Start page: 189;
Date: 2012;
Original page

Keywords: T | vaginalis | metabolic pathway | genome evolution | lateral gene transfer.

ABSTRACT
Trichomonas vaginalis causes the trichomoniasis, in women and urethritis and prostate cancer in men. Its genome draft published by TIGR in 2007 presents many unusual genomic and biochemical features like, exceptionally large genome size, the presence of hydrogenosome, gene duplication, lateral gene transfer mechanism and the presence of miRNA. To understand some of genomic features we have performed a comparative analysis of metabolic pathways of the T. vaginalis with other 22 common organisms. Enzymes from the biochemical pathways of T. vaginalis and other selected organisms were retrieved from the KEGG metabolic pathway database. The metabolic pathways of T. vaginalis common in other selected organisms were identified. Total 101 enzymes present in different metabolic pathways of T. vaginalis were found to be orthologous by using BLASTP program against the selected organisms. Except two enzymes all identified orthologous enzymes were also identified as paralogous enzymes. 75 of identified enzymes were also identified as essential for the survival of T. vaginalis while 26 as non-essential. The identified essential enzymes also represent as good candidate for novel drug targets. Interestingly some of the identified orthologous and paralogous enzymes were found playing significant role in the key metabolic activities and others were found playing active role in the process of pathogenesis. The N-acetylneuraminate lyase was analyzed as the candidate of lateral genes transfer. These findings clearly suggest the active participation of lateral gene transfer and gene duplication during evolution of T. vaginalis from the enteric to the pathogenic urogenital environment.

Tango Jona
Tangokurs Rapperswil-Jona

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