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Interleukin-1 and TNF- polymorphisms and Helicobacter pylori in a Brazilian Amazon population

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Author(s): Hivana Patricia Melo Barbosa, Luisa Caricio Martins, Sidney Emanuel Batista dos Santos, Samia Demachki, Mônica Baraúna Assumpção, Charliana Damasceno Aragão, Tereza Cristina de Oliveira Corvelo

Journal: World Journal of Gastroenterology
ISSN 1007-9327

Volume: 15;
Issue: 12;
Start page: 1465;
Date: 2009;
Original page

Keywords: Helicobacter pylori | Interleukin 1β gene | Interleukin-1 receptor antagonist gene | TNF-α gene | Cag pathogenicity island

ABSTRACT
AIM: To study the association between Interleukin-1 (IL-1) and tumor necrosis factor (TNF)-α polymorphisms, infection by Helicobacter pylori (H pylori) and the development of gastrointestinal diseases.METHODS: Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP) and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS: A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL-1RN*2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL-RN*2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastric mucosa.CONCLUSION: Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.

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