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Intra-specific Genetic Diversity of Different Accessions of Cassia occidentalis by RAPD Markers

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Author(s): V Arya

Journal: Genetic Engineering and Biotechnology Journal
ISSN 2150-3516

Volume: 2011;
Start page: GEBJ-22;
Date: 2011;
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Keywords: RAPD | C. occidentalis | genetic diversity | medicinal plant

ABSTRACT
The RAPD technique has been successfully used in a variety of taxonomic and genetic diversity studies. The genetic diversity of ten accessions of C. occidentalis collected from different districts of Haryana has been reported in this paper using twelve random amplified polymorphic DNA primers. 111 bands were scored corresponding to an average of 9.25 bands per primer with 79 bands showing polymorphism (71.17%). Nine out of twelve primers gave more than 60% polymorphism. Jaccard similarity coefficient ranged from 0.54 to 0.73. A dendrogram constructed based on the UPGMA clustering method revealed two major clusters. Cluster-1 comprises of nine accessions which was further differentiated into two subclusters while Cluster–2 includes only accession C10. This study revealed rich genetic diversity among C. occidentalis accessions from Haryana region in India.
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