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Intracytoplasmic glutathione level in MII oocyte during in vitro maturation of germinal vesicle: Effect of cysteamine

Author(s): Mohammadi Roushandeh A. | Noori Mooghahi. M.H. | Pasbakhsh. P. | Abdolvahhab M. | Akbari.M. | Shokrgozar M.A. | Sobhani A. | Amidi F. | . Abbasi Habibi M. | Roudkenar M..

Journal: DARU : Journal of Pharmaceutical Sciences
ISSN 1560-8115

Volume: 15;
Issue: 1;
Start page: 12;
Date: 2007;
Original page

Keywords: Germinal Vesicle

In the present study the kinetics of glutathione (GSH) concentration during in vitro maturation in the presence of Cysteamine in culture medium was examined. Also the effects of different doses of Cysteamine on Germinal Vesicle Breakdown (GVBD) and MetaphaseII (MII) development were investigated. Germinal vesicle (GV) oocytes was obtained from ICR mouse and cultured in Tissue culture medium (TCM199) supplemented with 0, 50, 100, 200 and 500 µM cysteamine. Number of GVBD and MII oocytes were recorded at 4 and 24 hours after culture respectively. For GSH assay 5, 5’-dithio-bis (2-nitrobenzoic acid)-glutathione disulfide (DTNB-GSSG) reductase recycling assay was employed. Our results showed that 100 µm cysteamine can improve GVBD and MII development significantly higher than control group (P
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