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INULINASE PRODUCTION BY ASPERGILLUS NIGER MYCELIUM IMMOBILIZED ON POLYURETHANE FOAM IN A BIOREACTOR WITH ALTERNATIVE OXYGENATION

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Author(s): MARCIN SKOWRONEK, JAN FIEDUREK AND MARIUSZ TRYTEK

Journal: International Journal of Biotechnology Applications
ISSN 0975-2943

Volume: 3;
Issue: 2;
Start page: 80;
Date: 2011;
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Keywords: inulinase | hydrogen peroxide | oxygenation | immobilization | mycelium | polyurethane foam

ABSTRACT
A system for the production of extracellular inulinase with the use of Aspergillus niger 20 Osm myceliumimmobilized on several polyurethane foam disks in a 2-liter bioreactor was developed. Because the application of the disksreduced the efficiency of the conventional aeration system initially employed in the study and caused difficulties inmaintaining sufficient concentrations of dissolved oxygen, an alternative culture oxygenation method based on O2generation by decomposition of sequential doses of hydrogen peroxide introduced into a bioreactor medium was used. Theapplication of the alternative oxygenation system during repeated batch culture made it possible to maintain adequateconcentrations of dissolved oxygen (50 – 99%) and a proper level of inulinase biosynthesis for a long time (up to 22 days).In the optimized conditions, the immobilized mycelium produced 86 321 units of inulinase activity during a 7-cycle process
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