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Investigate the Effect of Nd-Yag Laser Beam on Soybean (Glycin max) Leaves at the Protein Level

Author(s): Noha S. Khalifa | H. El Ghandoor

Journal: International Journal of Biology
ISSN 1916-9671

Volume: 3;
Issue: 2;
Date: 2011;
Original page

The effect of pre-sowing laser treatment on soybean seeds was investigated, using neodymium-doped yttriumaluminium garnet (Nd-Yag) laser source at wave length lamda= 532nm. Soybean uniform seeds (Glycin max L. cv.Giza 111) were exposed to laser irradiation for 5, 10, 30, 60 and 120 minutes. The growth potential of 4 - weekoldseedlings was progressively enhanced with the increase in time durations of laser treatment. In this study,protein banding patterns of the leaf pools of the irradiated and non-irradiated control plants were investigated toelucidate the role of laser at the molecular level. These patterns showed proportional increased levels of a ~ 55KD protein in alliance with the pre - sowing exposure time to laser irradiation. This protein was gel eluted andidentified using de novo protein sequencing analysis of Liquid Chromatography - Mass Spectrometry andTandem Mass Spectrometry (LC/ MS/MS) technique. The database search of the results led to identification ofthis protein as the large subunit of the enzyme 1,5 ribulose bisphosphate carboxylase/ oxygenase (RubisCO). Theconcomitantly enhanced growth potential of soybean seedlings with the elevation of the level of RubisCO willbe discussed from different points of views.
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