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Investigation of antiradical potential of different kinds of teas and extracts from these teas using antiradical activity units (TAU)

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Author(s): Damian Wojciechowski | Zbigniew Sroka | Andrzej Gamian

Journal: Postępy Higieny i Medycyny Doświadczalnej
ISSN 0032-5449

Volume: 65;
Issue: 846636;
Start page: 796;
Date: 2011;
Original page

Keywords: tea leaves | phenolic compounds | antiradical properties | liście herbaty | związki fenolowe | właściwości przeciwwolnorodnikowe

ABSTRACT
Introduction:Green, black and pu-erh teas are known to have strong antiradical properties in comparison to other plant raw materials.In this study fifteen different teas belonging to three types, green, black, and pu-erh tea (five of each kind), were investigated for their antiradical properties. Antiradical activity of extracts and raw materials was measured using DPPH and ABTS• radicals.Material/Methods:The antiradical potential of teas was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS• (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt). The TAU515 and TAU734 (antiradical activity units) were defined with the tests of DPPH and ABTS• , respectively, and the number of units was calculated per mg of extracts (TAU515/mg and TAU734/mg) and g of raw materials (TAU515/g and TAU734/g).Results/Discussion:When the extracts were investigated, the highest number of antiradical units (TAU515/mg; TAU734/mg) was found per mg of ethyl acetate extract obtained from green tea leaves assayed with DPPH and ABTS• : 57.7±0.8; 106±2.0, units respectively. When the number of antiradical units TAU515/g was calculated per g of raw material, the highest antiradical potential (7601±92) was observed for green tea leaves. The greatest TAU734/g value (14 303±354) was also calculated for green tea leaves. The lowest value of antiradical activity units TAU515/g (684±30) and TAU734/g (1870±180) was calculated for pu-erh tea leaves.A high positive correlation was found between the antiradical activity of the raw materials and the content of phenolic compounds in these raw materials.
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