Academic Journals Database
Disseminating quality controlled scientific knowledge

An investigation of a defensive chitinase against Fusarium oxysporum in pepper leaf tissue

Author(s): Khemika S. Lomthaisong

Journal: Maejo International Journal of Science and Technology
ISSN 1905-7873

Volume: 2;
Issue: 01;
Start page: 150;
Date: 2008;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: chitinase | isozyme | Fusarium oxysporum | PR-proteins | Capsicum annuum

Plant chitinase is classified as a PR-protein involved in a defense mechanism against a pathogen. This research aims to investigate a specific type of chitinase which is produced by pepper in response to an early defense against Fusarium oxysporum, which causes wilt disease. The changes of chitinase isozyme patterns in the inter- and intracellular fluids in the leaf of four cultivars of pepper (Capsicum annuum L.) at day 1, 3, 5, 7 and 10 from fungal inoculation were analysed using SDS-PAGE in polyacrylamide gel supplemented with glycol chitin as a substrate. The levels of disease severity in the four varieties of pepper were also compared with the isozyme patterns. The results showed that the resistance of pepper to F. oxysporum attack corresponded to the expression of ~70 kDa chitinase band (Chi-3) in the intercellular fluid. Therefore, such chitinase could possibly be used as a protein marker to identify the tolerant line and as a springboard for further study of wilt disease control.
Affiliate Program      Why do you need a reservation system?