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Investigation of the Properties of Covalent Immobilized Anti-aflatoxin B1 Antibody on Membranes from Copolymer of Polyacrylamide-polyacrylonitrile

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Author(s): Lyubov Yotova | Nedka Trifonova | Terry Vrabcheva | Vasilka Mironova | Vasilena Chuchuranova

Journal: International Journal Bioautomation
ISSN 1314-1902

Volume: 14;
Issue: 3;
Start page: 187;
Date: 2010;
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Keywords: Aflatoxins | Polyclonal antibody | Immobilization | ELISA

ABSTRACT
Aflatoxins are toxic secondary metabolites produced by a number of different fungi (Aspergillus flavus, Aspergillus parasiticus), and can be present in a wide range of food and feed commodities. The most used methods for analysis of aflatoxins are thin-layer chromatography, high-performance liquid chromatography (HPLC), electrochemical immunoanalysis and microtitre plate enzyme-linked immunosorbent assay (ELISA). Membranes from copolymer of polyacrylamide-polyacrylonitrile have been prepared. These membranes were used as a matrix for a covalent binding of polyclonal anti-aflatoxin B1 antibody. ELISA was carried out with these membranes to prove successful immobilization of the antibody. It was done comparative analysis with ELISA between standard microtiter plate and our membranes with infected peanuts.
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