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Investigation of Terahertz Spectral Signatures of DNA of Pine Wood Nematode

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Author(s): Ling Jiang | Chun Li | Lin Huang | Zhenwei Zhang | Cunlin Zhang | Yunfei Liu

Journal: Advance Journal of Food Science and Technology
ISSN 2042-4868

Volume: 4;
Issue: 6;
Start page: 426;
Date: 2012;
Original page

Keywords: Absorption frequency | Bm nematode | Bx nematode | terahertz spectroscopy

ABSTRACT
In this study, we present the fingerprint characteristic about the identification of harmful Bursaphelenchus xylophilus (Bx) nematode and harmless Bursaphelenchus mucronatus (Bm) nematode by applying terahertz spectroscopic techniques. We measure the transmission of the Deoxyribose Nucleic Acid (DNA) of the Bx and the Bm samples and their corresponding Polymerase Chain Reaction (PCR) amplification segments of the DNA molecules by the Terahertz Domain Spectroscopy (THz-TDS) and the Fourier Transform Infrared spectroscopy (FTIR). The low-frequency absorption features measured by THz-TDS exhibits different absorption peaks (i.e., 0.07 and 0.11 THz) for the Bx and Bm samples respectively. The calculated relative refractive index of the Bm-PCR segment is found to be much smaller compared with other three samples. The higher frequency characteristic of the four samples measured by the FTIR spectroscopy shows similar absorption peaks, except that the Bm-DNA provides a smooth differential feature of absorption at 5.46 THz. These measurement results indicate theBm samples including DNA and PCR segment indeed have unique signature behaviors different from the Bx samples. It demonstrates that the terahertz spectroscopic technique is a useful method to distinguish the Bx and the Bm’s DNA and PCR segments by locating the absorption frequency.

Tango Jona
Tangokurs Rapperswil-Jona

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