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Involvement of Serine Threonine Protein Kinase, PknL, from Mycobacterium Tuberculosis H37Rv in Starvation Response of Mycobacteria

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Author(s): Harini Lakshminarayan | A. Rajaram | Sujatha Narayanan

Journal: Journal of Microbial & Biochemical Technology
ISSN 1948-5948

Volume: 01;
Issue: 01;
Start page: 030;
Date: 2009;
Original page

Keywords: Serine threonine protein kinase (STPK) | PknL | Starvation response | Glutamine metabolism | Nutrient uptake | HPLC | Scanning electron microscopic studies

ABSTRACT
The adaptation to nutrient depletion in bacteria involvesa highly organized series of intracellular events that enablethem to adapt to starvation conditions. The regulatoryeffect of serine threonine protein kinase, PknL, fromMycobacterium tuberculosis strain H37Rv was investigatedunder nutrient deprived conditions that simulate circumstancesleading to latency. Recombinant PknL was expressedin Mycobacterium smegmatis strain mc2155 in itswild type and mutant forms. In vitro growth kinetics experimentsrevealed that clone expressing active PknL hada significant growth advantage under nutrient limitingconditions. Experiments were conducted to ascertain thein silico predictions of the involvement of PknL in regulatingglutamine metabolism in mycobacteria. Furthermore,a role for PknL in cell wall biogenesis/cell divisionwas shown by scanning electron microscopy.
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