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Isolation and characterization of gypenosides from Gynostemma pentaphyllum

Author(s): LIU Hui-Min  | YANG Jing | CHEN Dao-Jin | PIAO Xiang-Lan

Journal: Shipin Anquan Zhiliang Jiance Xuebao
ISSN 2095-0381

Volume: 4;
Issue: 1;
Start page: 283;
Date: 2013;
Original page

Keywords: Gynostemma pentaphyllum | dammarane-type saponin | gypenoside XLVI | gypenoside LVI

Objective To establish a method for the isolation and characterization of gypenosides from Gynostemma pentaphyllum. Methods Gypenosides were isolated with chromatography using silica gel and reversed semi-preparative HPLC and identified with 1H-nuclear magnetic resonance spectrum, 13C- nuclear magnetic resonance spectrum and liquid chromatography-ion trap time of flight tandem mass spectrometry. Results The two constituents isolated from Gynostemma pentaphyllum were identified as dammarane-type saponins, gypenoside XLVI and gypenoside LVI, respectively. Conclusion Ggypenosides can be isolated effectively by silica gel chromatography and reversed semi-preparative HPLC.
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