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Isolation and Characterization of Polymorphic Microsatellite Loci from Metapenaeopsis barbata Using PCR-Based Isolation of Microsatellite Arrays (PIMA)

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Author(s): Tzen-Yuh Chiang | Tzong-Der Tzeng | Hung-Du Lin | Ching-Ju Cho | Feng-Jiau Lin

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 13;
Issue: 3;
Start page: 2763;
Date: 2012;
Original page

Keywords: microsatellite | management | PIMA | RAPD-PCR enrichment | Metapenaeopsis barbata

ABSTRACT
The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Overfishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as observed and expected heterozygosities in populations, ranging from 0.048 to 0.538, and 0.048 and 0.654, respectively, were detected. No deviation from Hardy–Weinberg expectations was detected at either locus. No significant linkage disequilibrium was detected in locus pairs. The polymorphic microsatellite loci will be useful for investigations of the genetic variation, population structure, and conservation genetics of this species.
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