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Isolation, characterization and thin-layer chromatography method development of clerosterol palmityl ester: a chemical marker for standardization of leaves of Clerodendrum phlomidis

Author(s): Muthu Kumaradoss Mohan Maruga Raja

Journal: Zhong Xi Yi Jie He Xue Bao
ISSN 1672-1977

Volume: 10;
Issue: 1;
Start page: 109;
Date: 2012;
Original page

Keywords: chromatography | thin-layer | Clerodendrum | plant extracts

OBJECTIVE: Clerosterol palmityl ester (CPE) is a unique clerosterol derivative isolated and characterized from the leaves of Clerodendrum phlomidis. Considering the uniqueness of this compound, the present study was planned to use CPE as a specific chemical marker and develop a new validated thin-layer chromatography (TLC) method for standardisation of C.phlomidis. METHODS: Separation and quantification of CPE were achieved by TLC using a mobile phase of petroleum ether (60 to 80 ℃) and ethyl acetate (95∶5, volume ratio) (Rf 0.64) on precoated silica gel 60F254 aluminium plates. Densitometric determination was carried out after derivatization with anisaldehyde sulphuric acid reagent in absorption mode at 527 nm. RESULTS: The calibration curve was linear in the concentration range of 100 to 500 ng/spot. The method was validated for precision, repeatability and accuracy. The proposed method was found to be simple, specific, precise, accurate, rapid and cost-effective. CONCLUSION: This TLC procedure may be used effectively for quantitative determination of CPE, identification of the plant and standardization of this plant or its derived products.
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