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ISOLATION OF GLYCOSIDE FROM THE SEED POWDER OF SYZIGIUM CUMINI (L).

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Author(s): Vitthalrao B Khyade

Journal: International Journal of Bioassays
ISSN 2278-778X

Volume: 1;
Issue: 12;
Start page: 207;
Date: 2012;
Original page

Keywords: Syzigium cumini | Glycoside | Rhamnose

ABSTRACT
The seeds of Syzygium cumini(L) were shade dried, powdered and subjected for soxhlet extraction for twenty four hours through ethanol solvent. The extractive was concentrated. And it was then partitioned using petrol, benzene, ether, ethyl acetate, acetone and methanol. The soluble fractions were separated. The acetone soluble fraction was subjected for Si-gel CC and gradiently eluted with ethyl acetate, acetone mixture in various concentrations. The elutes collected from ethyl acetate: acetone (9:2) were combined and crystallized from ether as a light yellow needles. This compound was dissolved in aqueous ethyl alcohol (1:1 v/v) and then treated with Tokadiastase. Liberation of free rhamnose was detected, suggesting the presence of alpha linkage between sugar moiety and aglycone. The resultant compound provided enough evidence regarding “5, 7-dihydroxy-6, 2-dimethoxyisoflavone-7-O-alpha-L-rhamnoside”.// //
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