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Isolation and Identification of Calystegines in Root Cultures of four Physalis Species

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Author(s): Mohammad Ebrahim Azemi | Mahmoud Mosaddegh | Abdol Majid Cheraghali | Forough Namjooyan | Birgit Dräger

Journal: Iranian Journal of Pharmaceutical Research
ISSN 1735-0328

Volume: 5;
Issue: 1;
Start page: 69;
Date: 2006;
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Keywords: Calystegine | polyhydroxy nortropane alkaloids | physalis | solanaceae | gas chromatography

ABSTRACT
Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P.philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 μg/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P.philadelphia only contains calystegine B2 and P. philadelphia root cultures solely consist of calystegine A3. Isolation and identification of calystegines have been achieved by ion exchange column chromatography and gas chromatography, using authentic samples.
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