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Isolation and partial characterization of anti cancer protein from Bacillus pumilus against HepG2 cancer cell line

Author(s): V.Shanmugaraju1*, N.Abirami2, A.Mahalakshmipriya1 and R.Revathy1

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 5;
Start page: 1533;
Date: 2011;
Original page

Keywords: Anticancer peptide | Anticancer protein | Antimicrobial peptides.

Bacillus pumilus was isolated from soil sample and confirmed by 16s rDNA sequencing. The anti cancer protein was obtained by ammonium sulphate precipitation and purified by Sephadox G100 column chromatography. The lyophilized fractions were screened for anti MRSA (Methicillin Resistant Staphylococcus aureus) and anti ancer activity against liver cancer cells (Hep G2) 150μg/ml of protein exhibited the high level of cyotoxicity (75%) towards the Hep G2 cells where the positive control cyclophospamide showed cytotoxicity of 76%. Theanti cancer protein was characterized by 2D gel electrophoresis and peptide Mass Fingerprinting using MALTI TOF MS.
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